Journal: Frontiers in Plant Science

Article Title: CRISPR-Cas9: Tool for Qualitative and Quantitative Plant Genome Editing

doi: 10.3389/fpls.2016.01740

Figure Lengend Snippet: Specific commercial products and services available to the researchers to implement CRISPR technology.

Article Snippet: Integrated DNA technologies (IDT) , Human HPRT PCR Primer Mix, Mouse HPRT PCR Primer Mix, Nuclease Free Duplex Buffer , S.p. Cas9 Expression Plasmid , S.p. Cas9 Nuclease 3NLS (100, 500 μg) , CRISPR Negative Control crRNA, CRISPR Positive Control crRNA.

Techniques: CRISPR, Genome Wide, Clone Assay, Stable Transfection, Selection, Transfection, Plasmid Preparation, Mutagenesis, Expressing, Negative Control, Positive Control, Construct, Knock-In, Multiplex Assay, Amplification, Sequencing

Journal: Cell reports

Article Title: Sarcomere function activates a p53-dependent DNA damage response that promotes polyploidization and limits in vivo cell engraftment

doi: 10.1016/j.celrep.2021.109088

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The custom sub-library containing a 2,032 sgRNA subset from the Brunello library targeting 483 genes and 100 controls was ordered from IDT, ligated into lentiGuide-Puro with NEB HiFi DNA Assembly, and amplified in Stbl4 E.coli (Invitrogen 11635018).

Techniques: Peptide Microarray, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, TUNEL Assay, Software, Recombinant, Transfection, Protease Inhibitor, Plasmid Preparation, CRISPR